Premium
A mouse bone marrow stromal cell line, TBR‐B, shows inducible expression of smooth muscle‐specific genes
Author(s) -
Arakawa Emi,
Hasegawa Kazuhide,
Yanai Nobuaki,
Obinata Masuo,
Matsuda Yuzuru
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01995-5
Subject(s) - calponin , stromal cell , microbiology and biotechnology , caldesmon , cellular differentiation , cell culture , bone marrow , actin , biology , gene expression , in vitro , chemistry , gene , immunology , biochemistry , cancer research , genetics , calmodulin , enzyme
We established an in vitro culture system which mimicked the differentiation pathway of smooth muscle cell, using TBR‐B, a bone marrow stromal cell line derived from transgenic mice harboring temperature‐sensitive SV40 large T‐antigen gene. TBR‐B cells have the potential to express smooth muscle‐specific genes including h1‐calponin, h‐caldesmon, SM22α and α‐actin, only after cultured in the differentiation medium for 2 weeks. The differentiation state of TBR‐B was well controlled by using different culture medium. Using this cell line, we also found that ascorbic acid is a potent factor inducing the expression of h1‐calponin and α‐actin. TBR‐B cells will serve as a useful tool for elucidating the regulatory mechanisms of smooth muscle‐specific gene expression, and for identifying compounds that regulate the differentiation state of vascular smooth muscle cells.