Premium
Expression of T‐ and L‐type calcium channel mRNA in murine sinoatrial node
Author(s) -
Bohn Georg,
Moosmang Sven,
Conrad Heinke,
Ludwig Andreas,
Hofmann Franz,
Klugbauer Norbert
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01979-7
Subject(s) - sinoatrial node , diastolic depolarization , depolarization , chemistry , calcium , heartbeat , medicine , endocrinology , messenger rna , voltage dependent calcium channel , calcium channel , biophysics , microbiology and biotechnology , heart rate , biology , biochemistry , gene , blood pressure , computer security , computer science
At the cellular level, cardiac pacemaking which sets the rate and rhythm of the heartbeat is produced by the slow diastolic depolarization. Several ion channels contribute to this pacemaker depolarization, including T‐type and L‐type calcium currents. To evaluate the molecular basis of the currents involved, we investigated the cellular distribution of various low voltage activated (LVA) and high voltage activated (HVA) calcium channel mRNAs in the murine sinoatrial (SA) node by in‐situ hybridization. The most prominently expressed LVA calcium channel in the SA node is Ca v 3.1, whereas Ca v 3.2 is present at moderate levels. The dominant HVA calcium channel transcript is Ca v 1.2; only traces of Ca v 1.3 mRNA are detectable in SA myocytes of mice.