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Cell‐cycle regulation of the p53‐inducible gene B99
Author(s) -
Collavin Licio,
Monte Martin,
Verardo Roberto,
Pfleger Cathie,
Schneider Claudio
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01969-4
Subject(s) - cell cycle , mitosis , microbiology and biotechnology , gene , biology , cell , gene expression , cell cycle progression , regulation of gene expression , genetics
B99 is a p53‐inducible gene whose accumulation upon p53 activation is restricted to late S/G2 cells. Here we have analyzed B99 regulation during the cell cycle in murine cells with or without functional p53. We report that B99 accumulates in late S/G2 phase, is phosphorylated in mitosis, and disappears in G1 phase, regardless of the status of p53. As a complement to this observation, we show that B99 is not induced by p53 in quiescent cells. Therefore, B99 expression is modulated both by cell‐cycle regulatory mechanisms and by p53, and p53 can increase the cellular levels of B99 only during the window of the cell cycle when it is normally expressed. On the basis of these observations we rename B99 Gtse‐1 (G‐two‐ and S‐phase‐expressed).