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Three new nuclear genes, sigD , sigE and sigF , encoding putative plastid RNA polymerase σ factors in Arabidopsis thaliana
Author(s) -
Fujiwara Makoto,
Nagashima Akitomo,
Kanamaru Kengo,
Tanaka Kan,
Takahashi Hideo
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01965-7
Subject(s) - biology , gene , arabidopsis , arabidopsis thaliana , plastid , intron , genetics , gene duplication , phylogenetic tree , rna polymerase , rna , gene family , genome , chloroplast , mutant
Three new nuclear genes ( sigD , sigE and sigF ) of Arabidopsis thaliana , encoding putative plastid RNA polymerase σ factors, were identified and analyzed. Phylogenetic analysis revealed that higher plant σ factors fell into at least four distinct subgroups within a diverse protein family. In addition, Arabidopsis sig genes contained conserved chromosomal intron sites, indicating that these genes arose by DNA duplication events during plant evolution. Transcript analyses revealed two alternatively spliced transcripts generated from the sigD region, one of which is predicted to encode a σ protein lacking the carboxy‐terminal regions 3 and 4. Finally, the amino‐terminal sequence of the sigF gene product was shown to function as a plastid‐targeting signal using green fluorescent protein fusions.