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Cells overexpressing fructose‐2,6‐bisphosphatase showed enhanced pentose phosphate pathway flux and resistance to oxidative stress
Author(s) -
Boada Jordi,
Roig Teresa,
Perez Xavier,
Gamez Antonio,
Bartrons Ramon,
Cascante Marta,
Bermúdez Jordi
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01950-5
Subject(s) - pentose phosphate pathway , oxidative stress , glycolysis , oxidative phosphorylation , fructose , pentose , biochemistry , flux (metallurgy) , chemistry , fructose 1,6 bisphosphatase , metabolism , microbiology and biotechnology , biology , fermentation , organic chemistry
Changes in the content of fructose‐2,6‐bisphosphate, a modulator of glycolytic flux, also affect other metabolic fluxes such as the non‐oxidative pentose phosphate pathway. Since this is the main source of precursors for biosynthesis in proliferating cells, PFK‐2/FBPase‐2 has been proposed as a potential target for neoplastic treatments. Here we provide evidence that cells with a low content of fructose‐2,6‐bisphosphate have a lower energy status than controls, but they are also less sensitive to oxidative stress. This feature is related to the activation of the oxidative branch of the pentose phosphate pathway and the increased production of NADPH.