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In vitro nuclear reconstitution could be induced in a plant cell‐free system
Author(s) -
Zhao Yun,
Liu Xiaoling,
Wu Min,
Tao Wei,
Zhai Zhonghe
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01938-4
Subject(s) - chromatin , microbiology and biotechnology , xenopus , cell nucleus , micrococcal nuclease , nuclear membrane , nuclear pore , nucleosome , nuclease , biology , sperm , biophysics , cytosol , cell free system , cell , inner membrane , in vitro , nucleus , dna , biochemistry , genetics , mitochondrion , gene , enzyme
A cell‐free system derived from carrot cell cytosol extract has been developed for reassembling nuclear structure around the added demembranated sperm chromatin of Xenopus . Morphological evidence suggests that reassembled nuclei display the typical characteristics of normal eukaryotic nuclei, such as double‐layered nuclear membrane and nuclear pores. Micrococcal nuclease treatment indicates that remodeling of the demembranated sperm chromatin has occurred and the structure of nucleosome is formed during nuclear reconstitution. These data indicate that the nuclear reconstitution can be induced in cell‐free systems from plants, and the self‐assembly of the nucleus is ubiquitous in both animal and plant cells.