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Differential gene expression in mesothelioma
Author(s) -
Rihn B.H.,
Mohr S.,
McDowell S.A.,
Binet S.,
Loubinoux J.,
Galateau F.,
Keith G.,
Leikauf G.D.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01913-x
Subject(s) - mesothelioma , gene , biology , gene expression , complementary dna , cell culture , gene expression profiling , cell , microarray , microarray analysis techniques , microbiology and biotechnology , cancer research , transformation (genetics) , genetics , pathology , medicine
To investigate the molecular events controlling malignant transformation of human pleural cells, we compared constitutive gene expression of mesothelioma cells to that of pleural cells. Using cDNA microarray and high‐density filter array, we assessed expression levels of >6500 genes. Most of the highly expressed transcripts were common to both cell lines and included genes associated with stress response and DNA repair, outcomes consistent with the radio‐ and chemo‐resistance of mesothelioma. Interestingly, of the fewer than 300 genes that differed between cell lines, most functioned in (i) macromolecule stability, (ii) cell adhesion and recognition, (iii) cell migration (invasiveness), and (iv) extended cell division. Expression levels of several of these genes were confirmed by RT‐PCR and could be useful as diagnostic markers of human mesothelioma.