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Cleavage of lamin‐like proteins in in vivo and in vitro apoptosis of tobacco protoplasts induced by heat shock
Author(s) -
Chen Hao-Ming,
Zhou Jun,
Dai Yao-Ren
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01912-8
Subject(s) - lamin , dna fragmentation , apoptosis , fragmentation (computing) , heat shock protein , microbiology and biotechnology , protoplast , biology , in vitro , hsp70 , protease , cleavage (geology) , apoptotic dna fragmentation , blot , chemistry , biochemistry , programmed cell death , nucleus , enzyme , ecology , paleontology , fracture (geology) , gene
Apoptosis in heat shock‐treated tobacco protoplasts was evidenced by DNA fragmentation, flow cytometric analysis and activation of caspase 3‐like protease. Furthermore, an in vitro apoptosis system was established which reproduced the apoptotic events. Western blotting analysis using an antibody against lamin A and C showed that in both in vivo and in vitro systems lamin‐like proteins were cleaved into a 35‐kDa fragment, and that lamin‐like protein degradation precedes DNA fragmentation. Moreover, we found a 22.8‐fold increase in caspase 6‐like activity in cytosol of heat‐treated protoplasts as compared with the control.