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Nuclear factor Y activates the human xanthine oxidoreductase gene promoter
Author(s) -
Martelin Eeva,
Palvimo Jorma J.,
Lapatto Risto,
Raivio Kari O.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01909-8
Subject(s) - chemistry , gene , xanthine , promoter , microbiology and biotechnology , biology , biochemistry , gene expression , enzyme
To study the regulation of the human xanthine oxidoreductase (XOR) gene, we cloned 1.9 kb of the promoter region. In reporter gene assays, a construct encompassing nucleotides between −142 to +42 conferred maximal basal activity of the XOR promoter in 293T cells, in comparison with shorter (−92 to +42) or longer (up to −1937 to +42) constructs. The promoter activity was low in NIH‐3T3 cells. The most active construct contained a putative CCAAT motif at −119 to −123. Electrophoretic mobility shift assays showed that this sequence binds the ubiquitous nuclear factor Y (NF‐Y). Mutation of the CCAAT motif (CTGAT) abolished the NF‐Y binding and considerably reduced the promoter activity. Our data suggest an important functional role for NF‐Y in the transcriptional activation of the human XOR gene.