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Carboxyl‐terminal propeptide of type I collagen (c‐propeptide) modulates the action of TGF‐β on MC3T3‐E1 osteoblastic cells
Author(s) -
Mizuno Morimichi,
Fujisawa Ryuichi,
Kuboki Yoshinori
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01900-1
Subject(s) - protein precursor , chemistry , type i collagen , transforming growth factor , terminal (telecommunication) , microbiology and biotechnology , biochemistry , endocrinology , biology , gene , telecommunications , computer science
Previously we found that the carboxyl‐terminal propeptide of type I collagen (c‐propeptide) is a major secretory protein of MC3T3‐E1 osteoblastic cells. In this study, we found that c‐propeptide suppresses collagen synthesis and alkaline phosphatase activity of MC3T3‐E1 osteoblastic cells at the early‐differentiated stage in a dose dependent manner. Mature osteoblasts did not respond to c‐propeptide. These findings imply that c‐propeptide modulates the function of osteoblasts at an early differentiation stage. Transforming growth factor‐β (TGF‐β) is stored in bone and released from bone matrix after the resorption by osteoclasts. We investigated the effect of c‐propeptide on the action of TGF‐β, and found that it enhanced the effect of TGF‐β. We conclude that c‐propeptide is a physiological modulator of TGF‐β in bone metabolism.