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Lactate transport in rat adipocytes: identification of monocarboxylate transporter 1 (MCT1) and its modulation during streptozotocin‐induced diabetes
Author(s) -
Hajduch Eric,
Heyes Richard R.,
Watt Peter W.,
Hundal Harinder S.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01889-5
Subject(s) - monocarboxylate transporter , glucose transporter , endocrinology , extracellular , medicine , transporter , chemistry , intracellular , adipocyte , symporter , cotransporter , intracellular ph , biochemistry , biology , adipose tissue , insulin , sodium , organic chemistry , gene
We have characterised L ‐lactate transport in rat adipocytes and determined whether these cells express a carrier belonging to the monocarboxylate transporter family. L ‐Lactate was taken up by adipocytes in a time‐dependent, non‐saturable manner and was inhibited (by ∼90%) by α‐cyano‐4‐hydroxycinnamate. Lactate transport was stimulated by 3.7‐fold upon lowering extracellular pH from 7.5 to 6.5 suggesting the presence of a lactate/proton‐cotransporter. Antibodies against ono arboxylate ransporter 1 (MCT1) reacted positively with plasma membranes (PM), but not with intracellular membranes, prepared from adipocytes. MCT1 expression was down‐regulated in PM of adipocytes from diabetic rats, which also displayed a corresponding loss (∼64%) in their capacity to transport lactate. The data support a role for MCT1 in lactate transport and suggest that changes in MCT1 expression are likely to have important implications for adipocyte lactate metabolism.