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Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target
Author(s) -
Ui-Tei Kumiko,
Zenno Shuhei,
Miyata Yuhei,
Saigo Kaoru
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01883-4
Subject(s) - rna interference , chinese hamster , luciferase , microbiology and biotechnology , rna , chinese hamster ovary cell , hamster , rna silencing , biology , gene , chemistry , cell culture , genetics , transfection
A sensitive cellular assay system for RNA interference was developed using the firefly luciferase gene as target. RNA interference was noted not only in Drosophila cultured cells but Chinese hamster cells (CHO‐K1) as well, although double‐stranded RNA required for the latter was 2500 times more than for the former. Cognate double‐stranded RNA as short as 38 bp was found to be still capable of inducing RNA interference in Drosophila cultured cells.