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Annexin XI may be involved in Ca 2+ ‐ or GTP‐γS‐induced insulin secretion in the pancreatic β‐cell
Author(s) -
Iino Satoshi,
Sudo Toshiki,
Niwa Tae,
Fukasawa Tatsuya,
Hidaka Hiroyoshi,
Niki Ichiro
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01877-9
Subject(s) - annexin a2 , annexin , insulin , cytoplasm , pancreatic islets , immunocytochemistry , secretion , immunoelectron microscopy , medicine , endocrinology , biology , microbiology and biotechnology , annexin a5 , chemistry , antibody , islet , flow cytometry , immunology
The aim of this study was to investigate possible involvement of annexin XI in the insulin secretory machinery. In fluorescence immunocytochemistry, annexin XI was found in the cytoplasm of pancreatic endocrine cells and a pancreatic β‐cell line, MIN6, in a granular pattern. MIN6 cells also possessed weak and diffused annexin XI immunoreactivity in the cytoplasm. Immunoelectron microscopy revealed annexin XI in the insulin granules. Insulin secretion from streptolysin‐O‐permeabilized MIN6 cells was inhibited by anti‐annexin XI antibody, when the release was stimulated by either Ca 2+ or GTP‐γS, but not by a protein kinase C‐activating phorbol ester. Inhibition of insulin release by anti‐annexin XI antibody was reproduced in permeabilized rat islets. These findings suggest that annexin XI may be involved in the regulation of insulin secretion from the pancreatic β‐cells.