Premium
The role of mitochondrial dysfunction in regulation of store‐operated calcium channels in glioma C6 and human fibroblast cells
Author(s) -
Waśniewska Magdalena,
Duszyński Jerzy
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01862-7
Subject(s) - calcium , mitochondrion , microbiology and biotechnology , membrane potential , fibroblast , calcium in biology , chemistry , calcium signaling , glioma , calcium pump , intracellular , biology , biophysics , biochemistry , cancer research , in vitro , atpase , organic chemistry , enzyme
The store‐operated calcium influx into electrically non‐excitable cells is greatly modified under the condition of deenergized mitochondria in situ. The rate of calcium influx into cells with empty intracellular calcium stores is greatly diminished when cells were pretreated with 2 μM carbonyl cyanide m ‐chlorophenylhydrazone (a mitochondrial uncoupler) or with 4 μM myxothiazol (an inhibitor of the respiratory chain). We demonstrate that this general phenomenon takes place in the case of transformed (glioma C6 and Ehrlich ascites tumor cells) as well as non‐transformed (human fibroblasts) cells. We also demonstrate that the deenergization of mitochondria affects the cellular calcium influx rate and not the calcium pump on the plasma membrane.