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Changes in intramitochondrial cardiolipin distribution in apoptosis‐resistant HCW‐2 cells, derived from the human promyelocytic leukemia HL‐60
Author(s) -
Garcia Fernandez Maria,
Troiano Leonarda,
Moretti Laura,
Pedrazzi Jessica,
Salvioli Stefano,
Castilla-Cortazar Inma,
Cossarizza Andrea
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01861-5
Subject(s) - cardiolipin , mitochondrion , apoptosis , inner mitochondrial membrane , clone (java method) , membrane potential , microbiology and biotechnology , chemistry , atp–adp translocase , distribution (mathematics) , cell culture , biochemistry , biology , membrane , phospholipid , genetics , gene , mathematical analysis , mathematics
Using a cytofluorimetric approach, we studied intramitochondrial cardiolipin (CL) distribution in HCW‐2 cells, an apoptosis‐resistant clone of human HL‐60 cells. In HL‐60, about 50% of total CL is distributed in the outer leaflet of mitochondrial inner membrane, while in HCW‐2 a significantly higher amount of CL (about 65%) is in that site. In basal conditions, HSW‐2 cells also show a reduced mitochondrial membrane potential even if they are able to proliferate as the parental line. Taking into account the complex functions that CL plays in the regulation of mitochondrial activity, it is likely that HCW‐2 could produce ATP utilizing more glycolytic pathways rather than mitochondrial respiratory chain.