Premium
Possible His to Asp phosphorelay signaling in an Arabidopsis two‐component system
Author(s) -
Urao Takeshi,
Miyata Shinichi,
Yamaguchi-Shinozaki Kazuko,
Shinozaki Kazuo
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01860-3
Subject(s) - arabidopsis , arabidopsis thaliana , histidine kinase , two component regulatory system , biology , complementary dna , saccharomyces cerevisiae , protein–protein interaction , two hybrid screening , biochemistry , histidine , yeast , kinase , microbiology and biotechnology , gene , amino acid , mutant
We have so far cloned a cDNA encoding a hybrid‐type histidine kinase (ATHK1), three cDNAs encoding phosphorelay intermediates (ATHP1–3), and four cDNAs encoding response regulators (ATRR1–4) from Arabidopsis thaliana . To determine which molecules constitute a His to Asp phosphorelay pathway, we examined protein–protein interactions between them using a pairwise yeast two‐hybrid analysis, as an initial step. We detected a specific interaction between ATHK1 and ATHP1. We further examined protein–protein interactions between ATHP1–3 and other histidine kinases. We detected interactions between ETR1 and all ATHPs, and between CKI1 and ATHP1 or ATHP2. Interestingly, ERS1 could not interact with any ATHPs. We also examined protein–protein interactions between ATHP1‐3 and ATRR1‐4. The results indicated that ATHP2 could interact with ATRR4, and that ATHP3 could interact with ATRR1 or ATRR4. However, ATHP1 could not interact with any ATRRs. On the basis of these results, we discuss the possible phosphorelay networks in an Arabidopsis two‐component system.