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Premium Inhibition of inducible nitric oxide synthesis by oxidized lipoprotein(a) in a murine macrophage cell line
Author(s)
Moeslinger Thomas,
Friedl Roswitha,
Volf Ivo,
Brunner Monika,
Koller Elisabeth,
Spieckermann Paul Gerhard
Publication year2000
Publication title
febs letters
Resource typeJournals
PublisherWiley-Blackwell
Increased plasma levels of human lipoprotein(a) (Lp(a)) are highly correlated with the development of atherosclerotic lesions. During our study, we investigated the effects of native and hypochlorite oxidized lipoprotein(a) (ox‐Lp(a)) on nitric oxide production by the inducible nitric oxide synthase (iNOS) in lipopolysaccharide/interferon stimulated mouse macrophages (J774A.1). Ox‐Lp(a) (0–2 μg/ml) induces a dose dependent inhibition of inducible nitric oxide synthesis. iNOS protein expression showed a dose dependent reduction as revealed by immunoblotting when cells were incubated with increasing amounts of ox‐Lp(a). Ox‐Lp(a) decreases iNOS mRNA synthesis as shown by reverse transcription‐polymerase chain reaction. Ox‐Lp(a) induced iNOS inhibition might contribute to the development of atherosclerotic lesions by reducing the anti‐atherogenic effects of nitric oxide.
Subject(s)biochemistry , biology , cell culture , chemistry , cholesterol , endocrinology , genetics , in vitro , interferon gamma , lipopolysaccharide , lipoprotein , lipoprotein(a) , macrophage , microbiology and biotechnology , nitric oxide , nitric oxide synthase , organic chemistry
Language(s)English
SCImago Journal Rank1.593
H-Index257
eISSN1873-3468
pISSN0014-5793
DOI10.1016/s0014-5793(00)01825-1

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