Premium
Small proline‐rich protein 1 is the major component of the cell envelope of normal human oral keratinocytes
Author(s) -
Lee Chong Heon,
Marekov Lyuben N.,
Kim Soo-Youl,
Brahim Jaime S.,
Park Myung Hee,
Steinert Peter M.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01806-8
Subject(s) - involucrin , buccal administration , microbiology and biotechnology , biology , annexin , proline , cell , keratinocyte , chemistry , biochemistry , amino acid , in vitro , bioinformatics
Oral keratinocytes of buccal and gingival tissues undergo a terminal differentiation program to form a protective epithelial barrier as non‐keratinized or parakeratinized stratified cells. We have examined the protein composition of cell envelopes (CEs) from normal human buccal and gingival tissues as well as keratinocytes from normal human gingival cells grown in culture. Biochemical and sequencing analyses reveal that the CEs contain 60–70% small proline‐rich protein 1a/b (SPR1a/b), together with smaller amounts of involucrin, annexin I and several other known CE proteins. The data imply a specialized role for SPR1 proteins in the unique barrier function requirements of oral epithelia.