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Density‐dependent induction of TNF‐α release from human monocytes by immobilized P‐selectin
Author(s) -
Koike Junzo,
Nagata Kisaburo,
Kudo Sanae,
Tsuji Tsutomu,
Irimura Tatsuro
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01765-8
Subject(s) - tumor necrosis factor alpha , adhesion , chemistry , p selectin , platelet , stimulation , monocyte , l selectin , microbiology and biotechnology , alpha (finance) , e selectin , cell adhesion , medicine , endocrinology , biochemistry , biology , platelet activation , receptor , construct validity , organic chemistry , nursing , patient satisfaction
P‐selectin purified from human platelets, when immobilized on a solid surface, induced monocytes to release tumor necrosis factor‐α (TNF‐α). The induction of TNF‐α release was dependent on the concentration of P‐selectin used for the immobilization, and the maximal stimulation was observed when the plate was coated with 0.3 μg/ml of P‐selectin. Use of either a higher or a lower concentration of P‐selectin for the plate‐coating was found to elicit less TNF‐α release, although the higher concentration of P‐selectin caused a stronger adhesion of HL‐60 leukemic cells. The expression of mRNA for TNF‐α roughly paralleled the TNF‐α secretion, as assessed by RT‐PCR. These results indicate that monocytes are activated by immobilized P‐selectin in a density‐dependent manner.