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Up‐regulation of gut‐enriched krüppel‐like factor by interferon‐γ in human colon carcinoma cells
Author(s) -
Chen Zhi Y,
Shie Jue-Lon,
Tseng Chi-Chuan
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01764-6
Subject(s) - krüppel , apoptosis , interferon gamma , microbiology and biotechnology , biology , gene , chemistry , cytokine , gene expression , immunology , biochemistry
Interferon‐γ (IFN‐γ) induces growth arrest and apoptosis of tumor cells but the mechanisms for these functions are unknown. Recently, gut‐enriched krüppel‐like factor (GKLF) was found to possess similar biological properties. Treatment of HT‐29 cells with IFN‐γ inhibited cell proliferation and induced apoptosis, the effect was found to associate with GKLF expression. IFN‐γ stimulates GKLF mRNA and protein levels in a dose‐ and time‐dependent manner and this process is independent of p53, occurs rapidly and does not require de novo protein synthesis indicating that GKLF is an immediate‐early IFN‐γ‐responsive gene. Moreover, overexpression of GKLF results in similar effect as IFN‐γ suggesting that GKLF may function as a downstream target of IFN‐γ.