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Cloning and characterization of rat casein kinase 1ϵ
Author(s) -
Takano Atsuko,
Shimizu Kimiko,
Kani Shuichi,
Buijs Ruud M.,
Okada Masato,
Nagai Katsuya
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01755-5
Subject(s) - suprachiasmatic nucleus , casein kinase 1 , casein kinase 2 , biology , cloning (programming) , nucleus , protein kinase a , microbiology and biotechnology , complementary dna , kinase , gene , biochemistry , mitogen activated protein kinase kinase , hypothalamus , endocrinology , programming language , computer science
Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1ϵ (CK1ϵ) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1ϵ and characterization of the protein products. The rCK1ϵ is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS‐7 cells.