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Patch‐clamp study of liver nuclear ionic channels reconstituted into giant proteoliposomes
Author(s) -
Guihard Gilles,
Proteau Sonia,
Payet Marcel Daniel,
Escande Denis,
Rousseau Eric
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01752-x
Subject(s) - vesicle , lipid bilayer , biophysics , chemistry , bilayer , conductance , membrane potential , membrane , biochemistry , biology , physics , condensed matter physics
Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope (NE) vesicles into planar lipid bilayer proceeded with low efficiency. NE vesicle reconstitution into proteoliposomes led to NIC observations by the patch‐clamp technique. Large conductance, voltage‐gated, K + ‐permeant and Cl − ‐permeant NICs were characterized. An 80–105‐pS K + ‐permeant NIC with conducting sub‐state was also recorded. Our data establish that NICs can be characterized upon reconstitution into giant proteoliposomes and retain biophysical properties consistent with those described for native NICs.