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Tissue‐specific expression of multiple mRNA variants of the mouse estrogen receptor α gene
Author(s) -
Kos Martin,
O'Brien Sinead,
Flouriot Gilles,
Gan Frank
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01750-6
Subject(s) - biology , messenger rna , microbiology and biotechnology , alternative splicing , gene , rna splicing , gene expression , untranslated region , estrogen receptor , three prime untranslated region , genetics , rna , breast cancer , cancer
The isolation of five new mouse estrogen receptor α mRNA variants (mERα A, B, F1, F2 and H) is described. All of these transcripts are generated by alternative splicing and all encode the 66 kDa ERα protein that the previously identified mRNA C variant generates. However, these transcripts differ in their 5′ untranslated regions. RT‐PCR and S1 nuclease protection assays revealed a tissue‐ and sex‐specific expression pattern of all variants. The C and F mRNA variants are the predominantly expressed mERα variants in mouse. The expression of mERα H mRNA is restricted to liver, although female mice produce around a five fold higher level of this transcript than males. Our results show that the mERα gene is a complex genomic unit in mice that exhibits alternative splicing which is regulated in a tissue‐specific manner.