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Role of reactive oxygen species and phosphatidylinositol 3‐kinase in cardiomyocyte differentiation of embryonic stem cells
Author(s) -
Sauer Heinrich,
Rahimi Gohar,
Hescheler Jürgen,
Wartenberg Maria
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01747-6
Subject(s) - wortmannin , embryoid body , ly294002 , trolox , microbiology and biotechnology , phosphatidylinositol , reactive oxygen species , nadph oxidase , embryonic stem cell , chemistry , kinase , biochemistry , biology , adult stem cell , oxidative stress , gene , antioxidant capacity
Cardiotypic development in embryonic stem cell‐derived embryoid bodies may be regulated by reactive oxygen species (ROS). ROS were generated by a NADPH oxidase‐like enzyme which was transiently expressed during the time course of embryoid body development. Incubation with either H 2 O 2 or menadione enhanced cardiomyogenesis, whereas the radical scavengers trolox, pyrrolidinedithiocarbamate and N ‐acetylcysteine exerted inhibitory effects. The phosphatidylinositol 3‐kinase (PI‐3‐kinase) inhibitors LY294002 and wortmannin abolished cardiac commitment and downregulated ROS in embryoid bodies. Coadministration of LY294002 with prooxidants resumed cardiomyocyte differentiation, indicating a role for PI‐3‐kinase in the regulation of the intracellular redox state.