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Evidence for the presence of a component of the Mn complex of the photosystem II reaction centre which is exposed to water in the S 2 state of the water oxidation complex
Author(s) -
Evans Michael C.W.,
Rich Anne M.,
Nugent Jonathan H.A.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01742-7
Subject(s) - photosystem ii , chemistry , aqueous solution , spectroscopy , photosynthesis , manganese , component (thermodynamics) , photochemistry , analytical chemistry (journal) , environmental chemistry , physics , organic chemistry , biochemistry , quantum mechanics , thermodynamics
The interaction of water oxidising photosystem II preparations with the aqueous environment has been investigated using electron spin echo envelope modulation spectroscopy in the presence of 2 H 2 O. The spectra show interaction of 2 H of 2 H 2 O with the preparation in the S 2 state. The component interacting with water decays during 1–4 weeks storage at 77 K. No interaction of water with the classical multiline S 2 Mn signal, which is more stable on storage at 77 K, was detected. The results show that a component of the water oxidation complex, possibly involving the Mn centre, is accessible to water and may be the water binding site for photosynthetic water oxidation.