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Structure of the Bordetella pertussis 1414 endotoxin
Author(s) -
Caroff Martine,
Brisson Jean-Robert,
Martin Adèle,
Karibian Doris
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01720-8
Subject(s) - bordetella pertussis , chemistry , deamination , mass spectrometry , bordetella , epitope , matrix assisted laser desorption/ionization , hydrolysis , whooping cough , lipopolysaccharide , microbiology and biotechnology , biochemistry , chromatography , antigen , bacteria , desorption , organic chemistry , virology , biology , immunology , enzyme , genetics , adsorption , vaccination
The endotoxin (lipopolysaccharide) of Bordetella pertussis , the agent of whooping cough, consists of a lipid A linked to a highly branched dodecasaccharide containing several acid and amino sugars. The elucidation of the polysaccharide structure was accomplished by first analyzing the structures of fragments obtained by hydrolysis and nitrous deamination and then piecing the fragments together. The fine structure of the antigenic distal pentasaccharide, presented here, was determined by chemical analyses as well as by high‐resolution nuclear magnetic resonance and mass spectrometry. The complete structure was reconstituted and confirmed by matrix‐assisted laser desorption/ionization mass spectrometry. The following structure was derived from the combined experimental data: The detailed structure combined with previously reported serological data now allows the synthesis of its epitopes for potential vaccines.