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Effect of DNA topology on plasmid DNA repair in vivo
Author(s) -
Park Jik-Young,
Ahn Byungchan
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01718-x
Subject(s) - dna supercoil , dna gyrase , topoisomerase , nucleotide excision repair , dna , dna repair , plasmid , dna damage , biology , endonuclease , dna clamp , microbiology and biotechnology , circular bacterial chromosome , chemistry , biochemistry , escherichia coli , dna replication , gene , reverse transcriptase , rna
Escherichia coli nucleotide excision repair (NER) is responsible for removing bulky DNA adducts by dual incisions of the UvrABC endonuclease. Although the activity of the UvrAB complex which can induce DNA conformational change is employed in NER, the involvement of DNA topology and DNA topoisomerases remains unclear. We examined the effect of topoisomerase inhibitions on a NER in vivo system. The repair analysis of intracellular plasmid revealed that the DNA damage on positive supercoils generated by gyrase inhibition remained unrepaired, whereas the DNA damage was repaired in topoisomerase I mutants. These results suggest that DNA topology affects the NER process and the removal of positive supercoils by gyrase is vital for the efficiency of the E. coli NER system.