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Transcriptional regulation of the Saccharomyces cerevisiae DAL5 gene family and identification of the high affinity nicotinic acid permease TNA1 ( YGR260w )
Author(s) -
Llorente Bertrand,
Dujon Bernard
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01698-7
Subject(s) - nicotinic agonist , biochemistry , saccharomyces cerevisiae , permease , yeast , major facilitator superfamily , biology , gene , mutant , chemistry , receptor
We have studied the transcript levels of YGR260w and YLR004c , two genes encoding members of the yeast Dal5p subfamily of the major facilitator family, and we show that they increase when extracellular nicotinic acid and thiamine, respectively, are absent. The deletion of YGR260w in a bna1 auxotrophic mutant for nicotinic acid prevents growth at low nicotinic acid concentration. This suggests that YGR260w is necessary for nicotinic acid import into the cell. The direct measurement of nicotinic acid uptake on whole cells demonstrates that YGR260w encodes the yeast high affinity nicotinic acid permease. Its apparent K m of 1.7 μM is low enough to allow the uptake of the low concentrations of nicotinic acid normally secreted by wild type cells.