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Regulation of UDP‐glucose:ceramide glucosyltransferase‐1 by ceramide
Author(s) -
Komori Hironobu,
Ichikawa Shinichi,
Hirabayashi Yoshio,
Ito Makoto
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01696-3
Subject(s) - ceramide , glucosyltransferase , chemistry , biochemistry , lipid signaling , microbiology and biotechnology , biology , enzyme , apoptosis
We report that the expression of mRNA and the activity of UDP‐glucose:ceramide (Cer) glucosyltransferase‐1 (GlcT‐1) of human hepatoma Huh7 and mouse melanoma B16 cells increases after treatment with bacterial sphingomyelinase or upon addition of short‐chain Cer. Interestingly, however, GlcT‐1 gene transcription was not increased by Cer when GlcT‐1 cDNA was introduced with the CMV promoter in GlcT‐1‐deficient GM95 cells, suggesting that the normal promoter region of GlcT‐1 gene is essential for the response. The conversion of C6‐Cer to C6‐GlcCer occurred much more rapidly in GlcT‐1‐overexpressing Huh7 cells than in mock transfectants. As a result, GlcT‐1‐overexpressing cells acquired a greater resistance to C6‐Cer‐mediated cell death.