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Aquaporin 3 cloned from Xenopus laevis is regulated by the cystic fibrosis transmembrane conductance regulator
Author(s) -
Schreiber Rainer,
Pavenstädt Hermann,
Greger Rainer,
Kunzelmann Karl
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01689-6
Subject(s) - cystic fibrosis transmembrane conductance regulator , xenopus , cystic fibrosis , regulator , microbiology and biotechnology , transmembrane protein , biology , aquaporin , chemistry , biochemistry , gene , genetics , receptor
The cystic fibrosis transmembrane conductance regulator (CFTR) is essential for epithelial electrolyte transport and has been shown to be a regulator of epithelial Na + , K + , and Cl − channels. CFTR also enhances osmotic water permeability when activated by cAMP. This was detected initially in Xenopus oocytes and is also present in human airway epithelial cells, however, the mechanisms remain obscure. Here, we show that CFTR activates aquaporin 3 expressed endogenously and exogenously in oocytes of Xenopus laevis . The interaction requires stimulation of wild type CFTR by cAMP and an intact first nucleotide binding domain as demonstrated for other CFTR–protein interactions.