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Molecular cloning and functional characterization of the zebrafish ATP‐gated ionotropic receptor P2X 3 subunit
Author(s) -
Egan Terrance M.,
Cox Jane A.,
Voigt Mark M.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01685-9
Subject(s) - ionotropic effect , zebrafish , protein subunit , molecular cloning , cloning (programming) , chemistry , gamma aminobutyric acid receptor subunit alpha 1 , microbiology and biotechnology , biology , receptor , biochemistry , computational biology , gene , g alpha subunit , peptide sequence , glutamate receptor , computer science , programming language
We describe a P2X subunit cloned from the zebrafish ( Danio rerio ) that is an orthologue of the mammalian P2X 3 subunit. Like the mammalian P2X 3 , this receptor desensitizes rapidly in the presence of agonist. However, it differs in that αβ‐meATP is a much less potent agonist than ATP and the antagonist TNP‐ATP is not active at low nanomolar concentrations. Similar to the rat P2X 3 subunit, the zebrafish subunit forms hetero‐oligomeric assemblies with the rat P2X 2 that possesses a phenotype distinct from either parent. This novel clone will provide an important basis for future experiments investigating the structure/function relationships of P2X subunit domains.