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Homogeneous longitudinal profiles and synchronous fluctuations of mitochondrial transmembrane potential
Author(s) -
Diaz Giacomo,
Falchi Angela Maria,
Gremo Fulvia,
Isola Raffaella,
Diana Andrea
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01683-5
Subject(s) - mitochondrion , depolarization , homogeneous , cytoplasm , biophysics , membrane potential , transmembrane protein , chemistry , physics , biology , microbiology and biotechnology , receptor , biochemistry , statistical physics
This study reports for the first time (a) the longitudinal profile of the transmembrane potential (mΔ ψ ) of single mitochondria using a Nernstian fluorescent probe and (b) the distribution of mΔ ψ fluctuations of mitochondria undergoing permanent depolarization. Our findings show that (1) mitochondria in different energetic conditions coexist in the same cell, (2) mΔ ψ is rather homogeneous along the entire length of single mitochondria, (3) mΔ ψ is not influenced by the surrounding cytoplasmic environment and (4) mΔ ψ fluctuations occur simultaneously in groups of mitochondria connected in a network. Taken together, these findings provide further evidence for a functional relationship between mitochondrial arrangement and energetic condition.