Premium
Chemical inhibitors: a tool for plant cell cycle studies
Author(s) -
Planchais Séverine,
Glab Nathalie,
Inzé Dirk,
Bergounioux Catherine
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01675-6
Subject(s) - cell cycle , microbiology and biotechnology , mitosis , cell cycle protein , restriction point , proteolysis , cell , cell cycle checkpoint , biology , plant cell , cyclin , biochemistry , chemistry , enzyme , gene
Synchrony provides a large number of cells at defined points of the cell cycle. Highly synchronised cells are powerful and effective tools for molecular analyses and for studying the biochemical events of the cell cycle in plants. Usually, plant cell suspensions can be synchronised by chemical agents, which arrest the cell cycle by acting on the driving forces of the cell cycle engine such as cyclin‐dependent kinase activity, enzymes involved in DNA synthesis or proteolysis of cell cycle regulators or by acting on the cell cycle apparatus (mitotic spindle). The specificity, reversibility and efficiency of each type of cell cycle inhibitor are described and related to their mode of action.