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Estimation of H 2 O 2 gradients across biomembranes
Author(s) -
Antunes Fernando,
Cadenas Enrique
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01638-0
Subject(s) - cytosol , jurkat cells , membrane , extracellular , peroxisome , chemistry , biophysics , enzyme , biochemistry , biology , t cell , immune system , gene , immunology
When cells are exposed to an external source of H 2 O 2 , the rapid enzymatic consumption of H 2 O 2 inside the cell provides the driving force for the formation of the gradient across the plasma and other subcellular membranes. By using the concepts of enzyme latency, the following gradients – formed after a few seconds following the exposure to H 2 O 2 – were estimated in Jurkat T‐cells: [H 2 O 2 ] cytosol /[H 2 O 2 ] peroxisomes =3; [H 2 O 2 ] extracellular /[H 2 O 2 ] cytosol =7. The procedure presented in this work can easily be applied to other cell lines and provides a quantitative framework to interpret the data obtained when cells are exposed to an external source of H 2 O 2 .