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Identification of alternative splicing variants of the β subunit of human Ca 2+ /calmodulin‐dependent protein kinase II with different activities
Author(s) -
Wang Ping,
Wu Ya-Lan,
Zhou Tian-Hua,
Sun Yue,
Pei Gang
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01634-3
Subject(s) - alternative splicing , calmodulin , protein subunit , rna splicing , protein kinase a , complementary dna , microbiology and biotechnology , biology , kinase , exon , biochemistry , chemistry , gene , enzyme , rna
The β subunit of human Ca 2+ /calmodulin‐dependent protein kinase II (β CaMKII) was identified by searching through an expressed sequence tag database and rapid amplification of cDNA 5′‐ends and was assigned to chromosome 7. Reverse transcription‐polymerase chain reaction and sequencing analysis identified at least five alternative splicing variants of β CaMKII (β, β6, βe, β′e, and β7) in brain and two of them (β6 and β7) were first detected in any species. When expressed in HEK 293 cells, the Ca 2+ /calmodulin‐dependent kinase activity of β7, the shortest variant, was much lower than that of either β (the longest one) or βe (the medium one), suggesting possible regulation of β CaMKII activity by alternative splicing.