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Detection of the absorption of glucose molecules by living cells using atomic force microscopy
Author(s) -
de Souza Pereira Ricardo
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01622-7
Subject(s) - glucose oxidase , chemistry , enzyme , membrane , atomic force microscopy , hexokinase , molecule , cantilever , absorption (acoustics) , biophysics , nanotechnology , biochemistry , materials science , biology , organic chemistry , glycolysis , composite material
A very small electrode (nanobiosensor) was constructed by immobilizing enzyme (glucose oxidase or hexokinase) on the surface of the cantilever of the atomic force microscope in order to detect the absorption of glucose molecules by living cells. If glucose is present, the nanobiosensor deflects, probably due to the reaction heat evolved in the process. Nanobiosensors built with inactivated enzyme or cantilevers without immobilized enzyme were not capable of producing this type of signal (deflection). This technique will be very useful in detecting the passage of specific molecules through a cell wall (or a cell membrane for other types of cells).