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TSH regulates a gene expression encoding ERp29, an endoplasmic reticulum stress protein, in the thyrocytes of FRTL‐5 cells
Author(s) -
Kwon O-Yu,
Park Soojung,
Lee Woonghee,
You Kwan-Hee,
Kim Ho,
Shong Minho
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01617-3
Subject(s) - endoplasmic reticulum , cycloheximide , messenger rna , medicine , endocrinology , gene expression , gene , chemistry , biology , microbiology and biotechnology , protein biosynthesis , biochemistry
This experiment was performed to evaluate the effect of thyroid‐stimulating hormone (TSH) on the endoplasmic reticulum resident 29 kDa protein (ERp29) gene expression in the thyrocytes of FRTL‐5 cells. Although ERp29 mRNA was constantly expressed, its expression began to increase remarkably from 10 −9 M TSH. On the other hand, the effect of TSH on the abundance of ERp29 mRNA started within 6 h and peaked at 8 h. Actinomycin D strongly blocked this effect while cycloheximide did not. The half‐life of ERp29 mRNA was about 4–4.5 h in the presence or absence of TSH that was not affected by the stability of ERp29 mRNA. The effect of TSH on the ERp29 gene expression was specific, while other growth factors (transferrin, insulin and hydrocortisone) did not alter its expression. Our data indicate for the first time that the expression of ERp29 is regulated transcriptionally by TSH in thyrocytes.