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Human cytomegalovirus immediate early proteins upregulate endothelial p53 function
Author(s) -
Wang Jun,
Marker Paul H,
Belcher John D,
Wilcken David E.L,
Burns Linda J,
Vercellotti Gregory M,
Wang Xing L
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01604-5
Subject(s) - transfection , downregulation and upregulation , cytoplasm , microbiology and biotechnology , apoptosis , human cytomegalovirus , biology , plasmid , chemistry , virology , cell culture , gene , virus , biochemistry , genetics
Infected endothelial cells are found to be resistant to apoptosis possibly mediated by p53 cytoplasmic sequestration. We explored whether the immediate early 84 kDa protein (IE84) of cytomegalovirus (CMV) is responsible for p53 cytoplasmic sequestration. The endothelial cells were transfected with plasmids containing IE1 and 2 coding regions which are known to synthesize IE84 and 72 proteins. Our study found that p53 expression was significantly elevated in endothelial cells transfected with IE1 and 2 plasmids. However, p53 was only found in the nucleus rather than sequestered in the cytoplasm. We have demonstrated that IE84 and 72 are not responsible for p53 dysfunction caused by CMV infection, rather they upregulate p53 function and promote endothelial apoptosis.