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Biochemical characterization of the Drosophila Axin protein
Author(s) -
Yanagawa Shin-ichi,
Lee Jong-Seo,
Matsuda Yukihiro,
Ishimoto Akinori
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01601-x
Subject(s) - armadillo , wnt signaling pathway , dishevelled , biology , gsk 3 , microbiology and biotechnology , scaffold protein , transcription factor , drosophila (subgenus) , catenin , kinase , signal transduction , genetics , gene , frizzled
In the Wnt/Wingless pathway, accumulation of β‐catenin/Armadillo protein is a key regulatory step. Vertebrate Axin is a negative regulator of Wnt signaling, promoting glycogen synthase kinase‐3β‐mediated phosphorylation of β‐catenin and thereby destabilizing β‐catenin. Using Drosophila cell culture systems, we demonstrated that a Drosophila homolog of Axin (Daxin) inhibits Wingless‐induced Armadillo accumulation and Drosophila T‐cell factor‐dependent transcription induced by Wingless, Dishevelled, and Armadillo. The carboxy‐terminal portion of Daxin is not essential for the inhibitory activity, but a mutant only consisting of this portion behaves as a dominant‐negative protein. Moreover, interactions between Daxin and Zeste‐white 3, Armadillo, Dishevelled, protein phosphatase 2A and Daxin itself were shown, providing direct evidence that Daxin is a scaffold protein in the Wingless pathway.