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A novel fluorescent probe diphenyl‐1‐pyrenylphosphine to follow lipid peroxidation in cell membranes
Author(s) -
Okimoto Yuko,
Watanabe Akira,
Niki Etsuo,
Yamashita Takashi,
Noguchi Noriko
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01587-8
Subject(s) - hydrogen peroxide , lipid peroxidation , membrane , chemistry , phosphatidylcholine , fluorescence , liposome , membrane lipids , biochemistry , photochemistry , phospholipid , antioxidant , physics , quantum mechanics
Diphenyl‐1‐pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP=O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor with tert ‐butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12‐myristate 13‐acetate, which is known to produce several kinds of free radicals. It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically.