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Novel intracellular effects of human connective tissue growth factor expressed in Cos‐7 cells
Author(s) -
Kubota Satoshi,
Hattori Takako,
Shimo Tsuyoshi,
Nakanishi Tohru,
Takigawa Masaharu
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01573-8
Subject(s) - ctgf , connective tissue , growth factor , intracellular , microbiology and biotechnology , mitosis , cell growth , chemistry , biology , biochemistry , genetics , receptor
To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. In Cos‐7 cells, expression of human CTGF induced an altered cell morphology. It was associated with an increased cellular DNA content and loose attachment, indicating the cells were in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly. These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery.