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The Rab3‐interacting molecule RIM is expressed in pancreatic β‐cells and is implicated in insulin exocytosis
Author(s) -
Iezzi Mariella,
Regazzi Romano,
Wollheim Claes B.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01572-6
Subject(s) - exocytosis , blot , secretion , effector , transfection , microbiology and biotechnology , cell culture , biology , chemistry , endocrinology , gene , biochemistry , genetics
The putative Rab3 effector RIM (Rab3‐interacting molecule) was detected by Northern blotting, RT‐PCR and Western blotting in native pancreatic β‐cells as well as in the derived cell lines INS‐1E and HIT‐T15. RIM was localized on the plasma membrane of INS‐1E cells and β‐cells. An involvement of RIM in insulin exocytosis was indicated by transfection experiments of INS‐1E cells with the Rab3 binding domain of RIM. This domain enhanced glucose‐stimulated secretion in intact cells and Ca 2+ ‐stimulated exocytosis in permeabilized cells. Co‐expression of Rab3A reversed the effect of RIM on exocytosis. These results suggest an implication of RIM in the control of insulin secretion.