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Identification and light‐induced expression of a novel gene of NADPH‐protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana
Author(s) -
Oosawa Naoki,
Masuda Tatsuru,
Awai Koichiro,
Fusada Naoki,
Shimada Hiroshi,
Ohta Hiroyuki,
Takamiya Ken-ichiro
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01568-4
Subject(s) - protochlorophyllide , arabidopsis thaliana , gene isoform , chloroplast , biology , etiolation , oxidoreductase , biochemistry , biogenesis , arabidopsis , gene , microbiology and biotechnology , enzyme , mutant
In Arabidopsis thaliana , we identified a novel gene of a NADPH‐protochlorophyllide oxidoreductase (POR) isoform, which catalyzes the light‐dependent protochlorophyllide a reduction in the chlorophyll (Chl) biosynthetic pathway. The deduced amino acid sequence of the novel POR isoform (PORC) showed significant identities (∼75%) with the previously isolated two POR isoforms of A. thaliana . Contrasting with these POR isoforms, the PORC transcript increased in etiolated seedlings by illumination, and was dominantly expressed in immature and mature tissues. The present results demonstrated that Chl biosynthesis and chloroplast biogenesis in A. thaliana are controlled by three POR isoforms, which are differentially controlled by light and development.