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Novel Dictyostelium unconventional myosin, MyoM, has a putative RhoGEF domain
Author(s) -
Oishi Noboru,
Adachi Hiroyuki,
Sutoh Kazuo
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01564-7
Subject(s) - dictyostelium , pleckstrin homology domain , biology , myosin , microbiology and biotechnology , guanine nucleotide exchange factor , homology (biology) , coiled coil , motor protein , genetics , gtpase , gene , signal transduction , microtubule
We have cloned a novel unconventional myosin gene myoM in Dictyostelium . Phylogenetic analysis of the motor domain indicated that MyoM does not belong to any known subclass of the myosin superfamily. Following the motor domain, two calmodulin‐binding IQ motifs, a putative coiled‐coil region, and a Pro, Ser and Thr‐rich domain, lies a combination of dbl homology and pleckstrin homology domains. These are conserved in Rho GDP/GTP exchange factors (RhoGEFs). We have identified for the first time the RhoGEF domain in the myosin sequences. The growth and terminal developmental phenotype of Dictyostelium cells were not affected by the myoM − mutation. Green fluorescent protein‐tagged MyoM, however, accumulated at crown‐shaped projections and membranes of phase lucent vesicles in growing cells, suggesting its possible roles in macropinocytosis.