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Molecular characterization of a calmodulin‐like Dictyostelium protein CalB
Author(s) -
Rösel Daniel,
Půta František,
Blahůšková Anna,
Smýkal Petr,
Folk Petr
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01551-9
Subject(s) - calmodulin , dictyostelium discoideum , egta , ef hand , dictyostelium , gene , chemistry , biochemistry , null cell , molecular mass , microbiology and biotechnology , biology , enzyme , calcium , organic chemistry
A gene named calB was cloned and characterized in Dictyostelium . A relationship to calmodulin (CaM) is suggested by sequence identity (50%), similar exon–intron structure and cross‐reactivity with anti‐CaM sera. The level of calB mRNA is developmentally regulated with maxima during aggregation and in spores. CalB null cells grow normally, develop and produce viable spores. We demonstrated the capacity of tagged CalB to bind Ca 2+ using the 45 Ca 2+ overlay assay and showed that its mobility on SDS–PAGE is dependent on Ca 2+ /EGTA pretreatment.