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Cloning and expression analysis of an inducible HSP70 gene from tilapia fish
Author(s) -
Molina Alfredo,
Biemar Frédéric,
Müller Ferenc,
Iyengar Arati,
Prunet Patrick,
Maclean Norman,
Martial Joseph A.,
Muller Marc
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01538-6
Subject(s) - zebrafish , biology , tilapia , gene , hsp70 , reporter gene , gene expression , microbiology and biotechnology , oreochromis , carp , cloning (programming) , oreochromis mossambicus , nile tilapia , regulation of gene expression , heat shock protein , genetics , fish <actinopterygii> , fishery , computer science , programming language
We isolated and characterized the tilapia ( Oreochromis mossambicus ) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (−851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue‐independent gene expression in fish.