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Evidence that 3′‐phosphorylated polyphosphoinositides are generated at the nuclear surface: use of immunostaining technique with monoclonal antibodies specific for PI 3,4‐P 2
Author(s) -
Yokogawa Tohei,
Nagata Satoshi,
Nishio Yuichiro,
Tsutsumi Tomoaki,
Ihara Sayoko,
Shirai Ryuichi,
Morita Koji,
Umeda Masato,
Shirai Yasuhito,
Saitoh Naoaki,
Fukui Yasuhisa
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01535-0
Subject(s) - immunostaining , pi , phosphatidylinositol , monoclonal antibody , phosphorylation , microbiology and biotechnology , phosphatidylinositol 4,5 bisphosphate , signal transduction , stimulation , kinase , nucleus , staining , chemistry , biology , antibody , biochemistry , immunohistochemistry , endocrinology , immunology , genetics
Phosphatidylinositol (PI) 3,4‐P 2 is a phosphoinositide that has been shown to be important for signal transduction in growth factor stimulation. We have produced monoclonal antibodies specific for PI 3,4‐P 2 , which were able to detect PI 3,4‐P 2 generated in 293T cells treated with H 2 O 2 , or in MKN45/BD110 cells expressing activated PI 3‐kinase in immunostaining. Prolonged treatment with 0.05% Tween 20 resulted in detection of staining not only at the plasma membrane, but also at the nuclear surface, indicating that 3′‐phosphorylated phosphoinositides can be generated and function in the nucleus.