Premium
Mitochondrial DNA depletion causes morphological changes in the mitochondrial reticulum of cultured human cells
Author(s) -
Gilkerson Robert W,
Margineantu Daciana H,
Capaldi Roderick A,
Selker Jeanne M.L
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01527-1
Subject(s) - mitochondrial dna , mitochondrion , microbiology and biotechnology , reticular connective tissue , biology , mitochondrial apoptosis induced channel , ultrastructure , chemistry , inner mitochondrial membrane , biochemistry , anatomy , gene
Depletion of mitochondrial DNA (mtDNA) causes defects in respiratory activity and energy production. Recent studies have shown mitochondria to exist primarily as reticular networks, having tubular cristae. Using fluorescence microscopy and transmission electron microscopy, we have examined mitochondrial morphology and interior structure in wildtype and mtDNA‐depleted ρ0 human fibroblasts and 143B osteosarcoma cell lines. MtDNA depletion results in compromise of the mitochondrial continuum and causes a reduction in amount of cristal membranes, often prompting the remaining cristae to adopt a circular appearance in the mitochondrial interior. These changes emphasize the tight relationship between mitochondrial structure and function.