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Vascular endothelial growth factor (VEGF) directly enhances osteoclastic bone resorption and survival of mature osteoclasts
Author(s) -
Nakagawa Mari,
Kaneda Toshio,
Arakawa Toshiya,
Morita Shuichi,
Sato Takuya,
Yomada Takeo,
Hanada Koji,
Kumegawa Masayoshi,
Hakeda Yoshiyuki
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01520-9
Subject(s) - angiogenesis , bone resorption , vascular endothelial growth factor , growth factor , microbiology and biotechnology , endocrinology , chemistry , medicine , resorption , vascular endothelial growth factor a , biology , cancer research , receptor , vegf receptors
In bone development and regeneration, angiogenesis and bone/cartilage resorption are essential processes and are closely associated with each other, suggesting a common mediator for these two biological events. To address this interrelationship, we examined the effect of vascular endothelial growth factor (VEGF), the most critical growth factor for angiogenesis, on osteoclastic bone‐resorbing activity in a culture of highly purified rabbit mature osteoclasts. VEGF caused a dose‐ and time‐dependent increase in the area of bone resorption pits excavated by the isolated osteoclasts, partially by enhancing the survival of the cells. Two distinct VEGF receptors, KDR/Flk‐1 and Flt‐1, were detectable in osteoclasts at the gene and protein levels, and VEGF induced tyrosine phosphorylation of proteins in osteoclasts. Thus, osteoclastic function and angiogenesis are up‐regulated by a common mediator such as VEGF.