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Cd(II), Pb(II) and Zn(II) ions regulate expression of the metal‐transporting P‐type ATPase ZntA in Escherichia coli
Author(s) -
Binet Marie R.B,
Poole Robert K
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01509-x
Subject(s) - escherichia coli , chemistry , atpase , biochemistry , metal , microbiology and biotechnology , enzyme , biology , gene , organic chemistry
ZntA is a cation‐translocating ATPase which exports from Escherichia coli Cd(II) and Pb(II), as well as Zn(II). The metal‐dependent ATP hydrolysis activity of purified ZntA was recently characterised and showed a specificity for Cd(II), Pb(II) and Zn(II). zntA expression has been reported to be up‐regulated primarily by Zn(II), mediated by the regulatory protein ZntR, belonging to the MerR transcriptional regulator family. In contrast to previous claims, we now show, using a Φ( zntA‐lacZ ) monolysogen, that Cd(II) is the most effective inducer of zntA , which is also induced significantly by Pb(II). The Cd(II)‐ and Pb(II)‐dependent transcriptional up‐regulation of zntA is also mediated by ZntR.